【Objective 】The rapid multiplex-PCR method was established to detect simultaneously four foodborne pathogenic bacteria viz., Salmonella spp., Escherichia coli O157∶H7, Shigella spp. and Staphylococcus aureus, in order to provide technical support for quality and safety detection of aquatic products. 【Method】LB broth medium was used as enrichment medium to culture four foodborne pathogenic bacteria viz., Salmonella spp., E. coli O157:H7, Shigella spp. and S. aureus. Meanwhile, four pairs of primers were desi...
【基金】
广西科技攻关项目(桂科攻1355010-3)
【更新日期】
2016-03-03
【分类号】
TS254.7
【正文快照】
(Guangxi Academy of Fishery Science,Nanning 530021,China)0引言【研究意义】近年来,我国因致病菌污染引起食品中毒事件频繁发生,食源性致病菌已成为影响食品安全的最主要因素之一(董庆利等,2015)。随着渔业经济的发展及食品加工技术的进步,我国水产品市场供应日趋丰富,消费
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