【摘要】 本研究以不同纤维直径的细毛羊皮肤组织中的毛囊作为试验材料,利用不同的提取方法、不同pH范围的IPG胶条以及不同的上样量,探索适用于细毛羊皮肤组织的双向电泳体系,建立细毛羊皮肤组织毛囊的2-DE凝胶图谱。凝胶图谱用ImageMaster 2DPlatinum软件自动检测蛋白点比较不同纤维直径的细毛羊差异蛋白。结果显示,TCA/丙酮抽提法最适合细毛羊毛囊组织总蛋白质的提取。上样量为100μL,选择18cm、pH 4~7的线性IPG胶条,得到质量较好的双向凝胶电泳图谱,有35个蛋白差异点,为后续优质细毛羊的蛋白质组学研究工作奠定基础。更多还原

【Abstract】 In use of hair follicles in skin tissue of fine-wool sheep with different fiber diameters as experimental material,and different extraction methods,IPG strips with different pH and different sample volume as experimental methods,this paper was aimed to explore two-dimensional electrophoresis system applied to skin tissue of the fine-wool sheep and establish 2-DE gel map of skin tissue in fine-wool sheep.Gel maps using ImageMaster 2DPlatinum software automatically detected protein points to compare different protein fiber diameter of fine-wool sheep differences.The results showed that the TCA/acetone extraction method was most suited to fine-wool sheep hair follicle tissue extraction of total protein.Choose to 18 cm,pH 4to 7linear IPG strip,we got better quality two-dimensional gel electrophoresis,got 35 protein variations among the different cantons for the follow-up work fine-wool sheep with high quality of the study of proteomics.更多还原