【摘要】 目的探讨细胞周期蛋白D1(CyclinD1)、p16蛋白在胆汁反流性胃炎(BRG)中的表达,探寻BRG与胃癌的相关性。方法应用过氧化物酶标记的SP染色法进行免疫组织化学染色以检测癌基因CyclinD1及抑癌基因p16蛋白在正常胃黏膜、BRG、癌旁组织、胃癌中的表达。结果 CyclinD1在正常胃黏膜、BRG、癌旁组织、胃癌中的阳性表达率逐渐升高,在BRG中的阳性表达率显著高于正常胃黏膜组织,差异有统计学意义(P<0.05)。p16在正常胃黏膜、BRG、癌旁组织、胃癌中的阳性表达率逐渐降低,在BRG中的阳性表达率显著低于正常胃黏膜组织,差异有统计学意义(P<0.05)。但CyclinD1、p16蛋白在BRG、癌旁组织中的表达差异无统计学意义(P>0.05)。结论 BRG可能有癌变倾向,且CyclinD1、p16蛋白可能参与了其向胃癌发生发展的过程。更多还原

【Abstract】 Objective This paper evaluated the expression of CyclinD1 and p16 in bile reflux gastritis and studied the relationships between bile reflux gastritis and gastric cancer.Methods The SP immunohistochemical method was used to detect the expression of CyclinD1 and p16 in normal gastric mucosa,bile reflux gastritis,para-carcinoma tissues and gastric cancer.Results The positive expression rate of CyclinD1 gradually increased in normal gastric mucosa,bile reflux gastritis,para-carcinoma tissues,gastric cancer in order.The expression of CyclinD1 in bile reflux gastritis was much higher than that in normal gastric mucosa(P<0.05).The positive expression rate of p16 gradually decreased in normal gastric mucosa,bile reflux gastritis,para-carcinoma tissues,gastric cancer in order.The expression of p16 in bile reflux gastritis was much lower than that in normal gastric mucosa(P<0.05).The expression of CyclinD1 and p16 between bile reflux gastritis and para-carcinoma tissues showed no significant difference(P >0.05).Conclusion Bile reflux gastritis may be precancerous lesions while CyclinD1 and p16 may be involved in the development from bile reflux gastritis to gastric cancer.更多还原