【摘要】 目的:检测小类泛素化修饰蛋白1(SUMO-1)及雌激素受体α(ERα)在子宫内膜癌中的表达情况及两者间的关系。方法:选取天津市第五中心医院2006年1月—2013年12月间收集的50例子宫内膜癌标本及35例正常子宫内膜标本,采用免疫组织化学方法检测SUMO-1蛋白和ERα蛋白在所有组织标本中的表达情况,免疫荧光化学方法检测两者在子宫内膜癌中的共定位情况,分析SUMO-1和ERα的表达与子宫内膜癌临床病理参数的关系。结果:SUMO-1蛋白在子宫内膜癌中的阳性表达率高于对照组,而ERα蛋白则相反,2组差异均有统计学意义(均P<0.05)。子宫内膜癌患者SUMO-1蛋白和ERα蛋白阳性表达率在不同年龄、不同绝经状态、不同组织学分级的患者中差异有统计学意义(均P<0.05),不同病理类型的患者2种蛋白的阳性表达率差异无统计学意义(P>0.05)。SUMO-1和ERα在子宫内膜癌中的表达具有较好的一致性(Kappa=0.875,Z=5.473,P=0.001)。结论:SUMO-1和ERα在子宫内膜癌中存在共表达关系,两者在子宫内膜癌发生和发展过程中可能发挥协同作用。更多还原

【Abstract】 Objective:To detect the expression level of small ubiquitin-like modifier protein-1(SUMO-1) and estrogen receptor alpha(ERα), and their correlation in endometrial carcinoma. Methods:50 cases of endometrial carcinoma samples and35 cases of normal endometrial samples were collected in our hospital from 2006 January to 2013 December. The expression of SUMO-1 and ERα were detected in all tissues by immunohistochemical method, and their co-localization expression was detected by immunofluorescence method. Then the relationship between SUMO-1 and ERα, and their express level and the clinicopathological parameters were analyzed respectively. Results:The positive rates of SUMO-1 protein in endometrial carcinoma was higher than that in control group, while the expression of ERα protein was the opposite, and the difference between the two groups was statistically significant(P<0.05). The positive rate of SUMO-1 and ERα in endometrial carcinoma patients were differ in different ages, menopausal status and different histological grades(all P<0.05), but the difference was not significant in different pathology subtypes(P >0.05). The expression of SUMO-1 and ERα protein had good consistency(Kappa=0.875, Z=5.473, P=0.001). Conclusions:SUMO-1 and ERα were co-localization in endometrial carcinoma, and may play a synergistic role in the process and development of endometrial carcinoma.更多还原