【摘要】 目的:检测子宫内膜异位症(endometriosis,EMs)患者DNA甲基转移酶1(DNA methyltransferase,DNMT1)及DNMT3B在血清与腹腔液中的表达,探讨EMs增殖期和分泌期存在的甲基化改变。方法:选取2013年7—12月在北京大学深圳医院妇科及计划生育科行宫腹腔镜手术,并证实为EMs的患者61例作为研究组,其中Ⅰ~Ⅱ期39例(A组),Ⅲ~Ⅳ期22例(B组),选取同期行宫腹腔镜手术证实为非EMs的27例患者作为对照组(C组)。采用酶联免疫吸附试验(ELISA)测定3组增殖期和分泌期患者DNMT1及DNMT3B在血清及腹腔液中的表达。结果:1B组增殖期患者DNMT1在血清中的表达高于A组和C组增殖期患者,A、B组增殖期患者DNMT3B在血清中的表达高于C组增殖期患者,差异均有统计学意义(P<0.01)。2A组增殖期患者DNMT3B在腹腔液中的表达高于B组和C组增殖期患者,差异有统计学意义(P<0.01)。33组分泌期患者DNMTs在血清及腹腔液中的表达差异均无统计学意义(P>0.05)。结论:增殖期时EMs体内甲基化改变明显,且EMs不同分期的甲基化状态的改变也有差异,提示增殖期DNA甲基化改变在EMs的发生发展过程中起重要作用。更多还原

【Abstract】 Objective:To study the methylation alteration in menstrual proliferative and secretory period of endometriosis through testing the expression level of DNMT1 and DNMT3 B in serum and peritoneal fluid. Methods:Choose the women received the hysteroscopy-laparoscopy surgery in Peking University Shenzhen Hospital from July 2013 to December 2013. The study group divided by 61 cases of endometriosis(EMs), with 39 cases of Ⅰ-Ⅱ stages(group A) and 22 cases of Ⅲ-Ⅳ stages(group B). The control group divided by 27 cases of no-endometriosis(group C). Use the enzyme linked immunoabsorbent assay method to test DNMT1 and DNMT3 B expression level in serum and peritoneal fluid. Results:1In the proliferative period,group B had higher DNMT1 expression in serum than group A and C, group A and B had higher DNMT3 B expression in serum than group C, both with a statistically significant difference(P<0.01). 2In the proliferative period, group A had higher DNMT3 B expression in peritoneal fluid than group B and C, with a statistically significant difference( P < 0. 01). 3 In the secretory period, there were no statistically significant difference of DNMTs expression in serum and peritoneal fluid among three groups(P >0.05). Conclusions:In the proliferative period,methylation obviously change in EMs,methylation differently change in different EMs stages,indicating that the DNA methylation alteration in the proliferative period play an important role in the genesis and development of EMs.更多还原