【摘要】 目的检测HBV感染者血清中白细胞介素-33(IL-33)及白细胞介素-18(IL-18)的水平,探讨其与乙肝患者病情发展中的关系及其临床意义。方法用酶联免疫吸附法(ELISA)检测110例HBV感染者(无症状携带者35例,慢性乙肝患者42例,重症乙肝患者33例)和36例正常对照血清IL-33及IL-18的水平,同时检测以上乙肝患者血清转氨酶ALT,分析IL-33、IL-18与ALT的关系。结果重症乙肝患者组与慢性乙肝患者组血清IL-33及IL-18水平显著高于正常对照组(P<0.001),无症状携带者与对照组比较差异无显著(P>0.05),且各组乙肝患者IL-33及IL-18水平与血清谷丙转氨酶(ALT)水平呈正相关(分别为r=0.568;r=0.612,P<0.01)。结论 IL-33及IL-18在HBV感染者中高水平表达,可能与炎症因子激活有关,在HBV感染免疫损伤中发挥重要作用。更多还原

【Abstract】 Objective To detect interleukin-33 and interleukin-18 levels in serum of HBV infection and explore their relationships with the progression of the disease in hepatitis B and their clinical significance.Methods 110 hepatitis B patients(including35cases of asymptomatic carriers,42 cases of patients with chronic hepatitis B patients 33 cases with severe hepatitis B and 36 cases of normal control serum were determined for the levels of IL-33,IL-18 by ELISA.At the same time transaminase(ALT)in patients of the hepatitis B serum were detected and the relationship between IL-33,IL-18 and ALT were analysed.Results IL-33 and IL-18 levels in the serum of severe hepatitis B patients and chronic hepatitis B patients were significantly higher than those in normal control group(P<0.001);However,IL-33 and IL-18 levels in asymptomatic carriers compared with the control group has no significant difference(P>0.05).And serum IL-33 and IL-18 levels were positively correlated with alanine aminotransferase(ALT)in hepatitis group of all above(r=0.568,r=0.612 respectively,P<0.01).Conclusion High level expression of IL-33 and IL-18 in HBV infection shows that HBV infection may be related to activation of inflammatory factors and IL-33,IL-18 play an important role in HBV immune injury.更多还原