【摘要】 目的探讨金属β-内酰胺酶(MBL)检测方法,为临床合理选择抗菌药物提供确切的依据。方法收集104株耐亚胺培南鲍曼不动杆菌(CRAB),以乙二胺四乙酸(EDTA)纸片增效法检测MBL表型,并与聚合酶链反应(PCR)检测MBL基因结果进行比较。结果应用PCR方法,在104株受试菌中检出MBL基因阳性菌21株;EDTA纸片增效法检测出24株阳性菌株,对比PCR实验结果,该方法的敏感度95.2%,特异度95.1%。结论 EDTA纸片增效法检测鲍曼不动杆菌的MBL方法简便、结果可靠、成本低廉,适合于基层医院临床微生物学实验室对产MBL鲍曼不动杆菌的初步鉴定。更多还原

【Abstract】 Objective To research a method for detecting metallo-β-lactamase(MBL)to provide the basis for rationally selecting antibacterial drugs in clinic.Methods A total of 104 strains of imipenem-resistant Acinetobacter baumanmii(IRAB)were collected.The MBL phenotype was detected by using the EDTA disk potentiation test and the detection results were compared with the PCR detection results of MBL.Results Among 104 strains of tested IRAB,21 strains were the MBL gene positive by using PCR;24strains were the MBL gene positive by using the EDTA disk potentiation test.Compared with the PCR detection results,the sensitivity of the EDTA disk potentiation test was 95.2% and the specificity was 95.1%.Conclusion The EDTA disk potentiation test for detecting MBL of Acinetobacter baumanmii is simple,credible in the detection results and cheap in cost,which is suitable for the preliminary identification of the MBL-producing Acinetobacter baumanmii in the clinical microbiology laboratory of the primary hospitals.更多还原