【摘要】 目的比较两种不同方法培养艾滋病(AIDS)患者淋巴结抗酸杆菌的阳性率,并探讨此类患者抗酸杆菌的耐药情况。方法选择该院53例AIDS患者作为研究对象,采集淋巴结,用仪器法(3D法)和L-J法进行抗酸杆菌培养及其耐药实验。结果53例AIDS患者淋巴结经两种方法培养及检测,共培养出24例阳性标本,阳性率45.28%,其中3D法培养阳性阳性率为45.28%(24/53),罗氏培养法阳性率为41.50%(22/53),两种方法阳性率比较差异无统计学意义(χ2=0.15,P>0.05)。药敏结果有9例出现耐药,耐药率为37.5%。结论 3D法与罗氏法相互结合可提高检出率,淋巴结抗酸杆菌的培养和药敏试验能为临床诊断AIDS/TB和指导用药提供重要依据。更多还原

【Abstract】 Objective To compare the positive rate of two methods culturing Mycobacterium tuberculosis in AIDS patients with lymph node tuberculosis,and analyze their drug resistance.Methods A total of 53 AIDS patients were in this study,the Mycobacterium tuberculosis were cultured and did drug sensitivity test by BacT/ALERT@3D(3D)system method and roche method.Results A total of 24 patients with culture positive in 53 cases,total positive rate was 45.28%,the positive rates of 3Dsystem method and roche method were 45.28%(24/53)and 41.50%(22/53)respectively,the difference between these two methods was significant(χ2=0.15,P>0.05).Conclusion 3Dsystem method combined roche method could improve the positive rate of culturing Mycobacterium tuberculosis,lymph node n/med tuberculosis bacterium culture and drug sensitive experiment could provide important basis for diagnosis and guiding clinical medication.更多还原