【摘要】 目的:建立HPLC法测定颈痛舒贴膏中人参皂苷Rg₁、人参皂苷Rb₁、龙血素A、龙血素B的含量。方法:采用Diamonsil C₁₈柱（200 mm×4.6 mm,5μm）,以水-乙腈为流动相,梯度洗脱,检测波长为203 nm,流速为1.0m L/min,柱温为30℃测量人参皂苷Rg1、人参皂苷Rb1的含量;采用Diamonsil C18（150mm×4.6mm,5μm）色谱柱,流动相为乙腈-1%冰醋酸（34∶66）,体积流量为1.2 m L/min,柱温为40℃,检测波长为280 nm测量龙血素A、龙血素B的含量。结果:人参皂苷Rg1、人参皂苷Rb1、龙血素A、龙血素B线性范围分别为0.286¹.43、0.097⁰.485、0.019 52⁰.146 4、0.015 04⁰.112 8μg;平均回收率分别为98.62%、96.72%、99.95%、96.53%,RSD分别为1.74%、1.88%、1.09%、0.776%。结论:建立的方法简便、重复性好,可用于颈痛舒贴膏中人参皂苷Rg1、人参皂苷Rb1、龙血素A、龙血素B的含量测定。更多还原

【Abstract】 Objective: To establish HPLC method of determining the contents of ginsenoside Rg₁, Rb₁,loureirin A and B inJingTongShu strapping. Methods: Diamonsil C₁₈ column（200 mm×4.6 mm, 5 μm） was adopted,mobile phase: water-acetonitrile, gradient elution, detection wavelength was 203 nm, flow rate: 1.0 m L/min, column temperature: 30℃ to detect the contents of ginsenoside Rg₁ and Rb₁; Diamonsil C₁₈（150 mm×4.6 mm, 5 μm） was adopted, mobile phase: acetonitrile-1% glacial acetic acid（34: 66）, volume flow: 1.2m L/min, column temperature: 40℃,detection wavelength: 280 nm, to detect the contents of loureirin A and B. Results: Linear ranges of ginsenoside Rg₁,Rb₁, loureirin A and B were from 0.286 to 1.43, 0.097 to 0.485, 0.019 52 to 0.146 4, 0.015 04 to 0.112 8 μg; average recovery rate were 98.62%, 96.72%, 99.95% and 96.53%. RSD was 1.74%, 1.88%, 1.09% and 0.776% respectively.Conclusion: The method is simple and reproducible, which could be used for content determination of ginsenoside Rg₁, Rb₁, loureirin A and B inJingTongShu strapping.更多还原