【摘要】 目的研究消毒剂耐药基因qacEΔ1对常见革兰阴性(G-)菌耐药性的影响。方法对2013年7—12月某院新生儿科住院患儿送检标本分离的G-菌进行检测和药敏试验,应用聚合酶链反应(PCR)法对其qacEΔ1基因进行检测。结果共收集608株G-菌,以肺炎克雷伯菌(216株)居多,其次为大肠埃希菌(128株)、阴沟肠杆菌(77株)、鲍曼不动杆菌(62株)及铜绿假单胞菌(19株)。5种主要细菌qacEΔ1基因阳性率为62.75%(315/502),其中鲍曼不动杆菌qacEΔ1基因阳性率最高(87.10%)。主要细菌中,qacEΔ1基因阳性和阴性菌株对哌拉西林、头孢吡肟、氨曲南、亚胺培南、复方磺胺甲口恶唑的耐药率比较,差异有统计学意义(均P≤0.05)。5种主要细菌对哌拉西林、头孢他啶、头孢吡肟、氨曲南耐药率均高。结论新生儿病房中分离的5种主要G-菌耐消毒剂基因阳性率高,qacEΔ1基因阳性菌株对对哌拉西林、头孢吡肟、氨曲南、亚胺培南、复方磺胺甲口恶唑耐药率更高。更多还原

【Abstract】 Objective To study the effect of disinfectant resistance gene qacEΔ1 on the drug resistance of commonly isolated gram-negative bacteria.Methods Gram-negative bacteria isolated from neonates in a neonatal intensive care unit(NICU)in July-December 2013 were detected and performed antimicrobial susceptibility testing,qacEΔ1 gene were detected by polymerase chain reaction.Results A total of 608 gram-negative bacteria were collected,the major isolates were Klebsiella pneumoniae(n=216),followed by Escherichia coli(n=128),Enterobacter cloacae(n=77),Acinetobacter baumannii(n=62),and Pseudomonas aeruginosa(n=19).The positive rate of qacEΔ1 gene in above five bacteria was 62.75%(315/502),Acinetobacter baumannii was the highest(87.10%).qacEΔ1-positive and negative strains showed statistical difference in the resistance rates to piperacillin,cefepime,aztreonam,imipenem,and compound sulfamethoxazole(all P≤0.05).The resistance rate of five strains to piperacillin,ceftazidime,cefepime,and aztreonam were all high.Conclusion The positive rates of qacEΔ1 in five major gram-negative bacteria from neonates in NICU are all high,qacEΔ1-positive strains have even higher resistance rates to piperacillin,cefepime,aztreonam,imipenem,and compound sulfamethoxazole.更多还原