Inhibitive rate of conidia germination and spore germination of fungicide to Colletotrichum in navel orange were measured bygrowth rate method. Toxicity test in laboratory showed that prochloraz( 25%) had the best antibacterial effect with the minimum EC50 value of inhibited mycelial growth and spore germination. Asomate( 40%) and Azoxystrobin( 25%) inhibited mycelial growth significantly,while difenoconazole( 10%) inhibited spore germination significantly.