【摘要】 以Ce（Ⅳ）、1,4,7,10-四氮杂环十二烷（Cyclen）、叶绿素-Cu（Ⅱ）以及壳聚糖为原料,采用反相悬浮交联法和离子配位法制备的复合物（CCC[Ce（Ⅳ）,Cu（Ⅱ）]）,在60℃条件下,能够显著加速磷酯键的断裂,在60 min时该复合物对p NPP和AMP实时水解率分别为60%和165%,是p NPP自身水解速率的183倍,对应的表观反应速率常数Kobsd为1.9×10^-2M/min;是AMP自身水解速率的211倍,对应的表观反应速率常数Kobsd为3.9×10^-2M/min。该复合物可使p BR322 DNA从双螺旋结构完全转变为缺刻型及直线型,且缺刻型比例高达93%。更多还原

【Abstract】 CCC[Ce（ Ⅳ）,Cu（ Ⅱ） ] was prepared by attaching Ce（ Ⅳ）,1,4,7,10-tetraazacyclododecane and chlorophyll-Cu（ Ⅱ）,and using reversed-phase cross-linking method and ion binding method. The results indicated that p NPP and AMP was hydrolyzed up to 60% and 165% at 60 ℃ after 60 minutes. The pseudo-first-order kinetic constant（ Kobsd） was 1. 9 × 10^-2 M / min for p NPP treatment,3. 9 × 10^-2 M / min for AMP treatment. The complexes could significantly accelerate the cleavage of phosphoester bond. The time-hydrolysis rate was 183 times faster than p NPP itself,211 times faster than AMP itself. p BR322 DNA was transformed from the supercoiled to the nicked circle and the linear by CCC[Ce（ Ⅳ）,Cu（ Ⅱ） ],and the proportion of the nicked circles was as high as 93%.更多还原