【摘要】 目的观察长春西汀对慢性脑缺血大鼠学习记忆及海马区轴突再生抑制因子Nogo-A表达的影响。方法大鼠按照体重随机分为假手术组、模型组和实验组,取3,6,9周3个时间点,采用双侧颈总动脉阻断法建立慢性脑缺血模型。实验组术后给予长春西汀10 mg·kg^-1·d^-1,共21 d。Morris水迷宫检测认知功能,免疫组化及原位杂交检测不同时间点海马区Nogo-A表达。结果术后6周,假手术组、模型组、实验组的潜伏期分别为（14.76±4.34）,（33.23±3.42）,（27.74±5.30）s,跨越平台次数分别为（6.88±2.64）,（3.13±1.81）,（5.38±1.85）次,实验组大鼠较模型组潜伏期均缩短,跨越平台次数均增多（P<0.05）;与模型组相比,实验组在各时间点的海马CA1区Nogo-A、Nogo-A mRNA表达明显降低（P<0.05）。结论长春西汀可改善慢性脑缺血大鼠认知功能,这可能与其下调大鼠海马区Nogo-A有关。更多还原

【Abstract】 Objective To investigate the effect of vinpocetine on learning and memory function and the expression of Nogo- A in the hippocampus CA1 region of the rats with chronic cerebral ischemia.Methods The chronic cerebral ischemia rats model was induced by bilateral common carotid artery permanent occlusion.The rats were randomly divided into sham operation,model and experimental groups.Each group according to ischemic time was subdivided into 3,6 and 9 weeks groups.The rats in experimental group were injected vinpocetine intraperitoneally(ip by 10 mg·kg^-1),once a day for consecutive 14 d.Cognitive function of the rats were tested with the Morris water maze and the expression of Nogo- A was measured by immunohistochemistry and situ hibridization.Results After 6 weeks,the escape latency in the sham group,model group and the experimental group were（14.76±4.34）,（33.23±3.42）,（27.74±5.30） s,respectively.The number of cross-platform were（6.88±2.64）,（3.13±1.81）,（5.38±1.85）times,respectively.Compared with model group,the average escape latency significantly shortened,the number that rats passed through the platform significantly increased（P<0.05）,and the expression of Nogo- A protein and mRNA in hippocampal CA1 areas significantly increased（P<0.05） in the experiment group at different time points.Conclusion Vinpocetine can improve the cognitive dysfunction of chronic cerebral ischemia rats which might be correlated to the neuroprotective effect of vinpocetine on neuronal by down- regulating the expressions of Nogo- A protein and mRNA.更多还原