【摘要】 目的探讨结缔组织生长因子(CTGF)对脑胶质瘤细胞增殖的影响,并分析Rho激酶信号转导通路及雷公藤单体在其中的作用。方法取传代培养生长的脑胶质瘤细胞,按如下进行操作:正常对照组未予以任何无药物干预,CTGF组予以2.5μg·L-1CTGF孵育细胞24 h;雷公藤甲素组予以2.5μg·L-1CTGF与0.5 ng·m L-1雷公藤甲素共同孵育细胞24 h;雷公藤红素组予以2.5μg·L-1CTGF与0.5 ng·m L-1雷公藤红素共同孵育细胞24 h;Y27632(Rho激酶特异性抑制剂)组予以1μmol·L-1Y27632预处理细胞30 min后,加入2.5μg·L-1CTGF作用24 h。用酶联免疫吸附测定法对Rho激酶活性进行检测。用氚化胸腺嘧啶核苷法检测脑胶质瘤细胞的增殖。结果 CTGF组脑胶质瘤细胞DNA的合成量显著高于正常对照组(P<0.01),雷公藤甲素组和雷公藤红素组脑胶质瘤细胞DNA的合成量显著低于CTGF组(P<0.05),Y27632组脑胶质瘤细胞DNA的合成量显著低于CTGF组(P<0.05)。CTGF组的Rho激酶活性显著高于正常对照组(P<0.01),雷公藤甲素组和雷公藤红素组的Rho激酶活性显著低于CTGF组(P<0.05)。结论 CTGF可诱导脑胶质瘤细胞的增殖,雷公藤甲素和雷公藤红素可抑制CTGF诱导的脑胶质瘤细胞的增殖,Rho激酶信号转导通路参与CTGF诱导的脑胶质瘤细胞增殖。更多还原

【Abstract】 Objective To explore the role of Rho- kinase in glioma cell proliferation induced by connective tissue growth factor( CTGF) and effects of triptolide / tripterine. Methods A subculture growth of glioma cells were got to take the experiment,the normal control group was not give any drug intervention. CTGF group was given CTGF 2. 5 μg ·L- 1and incubation for 24 h. Triptolide group was given CTGF 2. 5 μg·L- 1and triptolide 0. 5 ng · m L- 1and incubation for 24 h. Tripterine group were given CTGF 2. 5 μg·L- 1and tripterine 0. 5 ng·m L- 1and incubation for 24 h. Y27632( group specificity of Rho kinase inhibitor) cells after 30 min to give CTGF 2. 5 μg·L- 1and Y27632 1 μmol·L- 1pretreatment. The expressions of Rho- kinase protein were detected by ELISA. Glioma cell proliferation was measured by3H- TdR assay.Results CTGF could induce the proliferation of glioma cell. Triptolide,tripterine and Y27632( Rho kinase inhibitor) could significantly reverse these effects( P < 0. 05). In addition,CTGF could induce the activationof Rho- kinase( P < 0. 01),while triptolide and tripterine could significantly reverse these effects( P < 0. 05),indicating the activation of Rho- kinase pathway participates in glioma cell proliferation induced by CTGF.Conclusion CTGF could induce the proliferation of glioma cell,while triptolide,tripterine and Y27632 could significantly reverse these effects. Rho- kinase pathway participates in glioma cell proliferation induced by CTGF.更多还原