【摘要】 目的制备猪脑神经肽(PBNC)并检测其对H2O2诱导的PC12细胞氧化损伤的保护效果。方法采用碱化-酶解法制备PBNC,高效液相色谱法测定PBNC分子量;利用H2O2诱导PC12细胞产生氧化损伤,MTT法检测细胞活性、流式细胞术检测细胞凋亡及活性氧水平。结果 PBNC中分子量为250~2 000的多肽约占81%。PBNC减轻了H2O2诱导的PC12细胞毒性作用,降低了凋亡率及活性氧生成。结论 PBNC对H2O2诱导的PC12细胞氧化损伤具有保护作用。更多还原

【Abstract】 Objective To prepare the porcine brain-derived neuropeptide compound（PBNC） and investigate its protective effect on H₂O₂-induced oxidative damage of PC12 cells. Methods PBNC was prepared by basification-enzymolysis method, and its molecular weight（Mr） was determined by HPLC. After the oxidative damage of PC12 cells was induced by H₂O₂, cell viability, apoptosis and reactive oxygen species（ROS） were determined by MTT and flow cytometry, respectively. ResultsApproximately 81% of PBNC were polypeptides with molecular weights of 250-2000. PBNC reduced the cytotoxicity, apoptosis and ROS production in H₂O₂-treated PC12 cells. Conclusion PBNC can be neuroprotective for H₂O₂-induced oxidative damage of PC12 cells.更多还原