【摘要】 目的建立与人类发病机理相似的大鼠口腔癌前病变与鳞癌模型,为进一步研究口腔鳞癌的发生发展机制提供实验对象。方法在屏障环境下将质量分数为0.004%的4-硝基喹啉-1-氧化物(4-nitro-quinoline-1-oxide,4NQO)水溶液,作为饮水给予大鼠自行饮用,观察大鼠口腔黏膜的变化,并在第10、14、18、22、24周分5批处死,采集口腔黏膜标本,确定病理分级及病变性质。结果 82只有效实验大鼠中:1舌正常黏膜25.6%(21/82)、上皮单纯增生25.6%(21/82)、轻度异常增生17.1%(14/82)、中度异常增生9.8%(8/82)、重度异常增生2.4%(2/82)、鳞癌19.5%(16/82);2腭正常黏膜20.7%(17/82)、上皮单纯增生23.2%(19/82)、轻度异常增生24.4%(20/82)、中度异常增生14.6%(12/82)、重度异常增生7.3%(6/82)、鳞癌9.8%(8/82)。实验组病理分级结果采用等级资料的多个独立样本的Kruskal-Wallis秩和检验,舌黏膜(χ2=20.738,P<0.005)、腭黏膜(χ2=15.003,P<0.005),结果分布差异均有统计学意义。结论该模型可排除其它外界因素对实验结果的干扰,实验效果明确,是理想的研究口腔鳞癌发生发展机制的动物模型。更多还原

【Abstract】 Objective To establish the oral precancerous and oral squamous cell carcinoma models which the pathogenesis is similar to human’s and provide experimental subjects for further study on the mechanism of occurrence and development of oral squamous cell carcinoma. Methods In barrier environment,4NQO aqueous solution with the mass fraction of 0. 004% was given to rats as water to drink. Then changes of oral mucosa in rats were observed. The rats were divided into 5 groups and each group of them was killed respectively in the 10 th week,14 th week,18 th week,22 th week and 24 th week. Oral mucosa specimens were collected and pathological classification was done to determine the nature of the lesions. Results Eighty-two cases of rats: 1 Tongue normal mucosa 25. 6%( 21 /82),epithelial hyperplasia25. 6%( 21 /82),mild dysplasia 17. 1%( 14 /82),moderate dysplasia 9. 8%( 8 /82),severe dysplasia 2. 4%( 2 /82)and squamous cell carcinoma1 9. 5%( 16 /82); 2 palatal normal mucosa 20. 7%( 17 /82),epithelial hyperplasia23. 2%( 19 /82),mild dysplasia 24. 4%( 20 /82),moderate dysplasia 24. 6%( 12 /82),severe dysplasia 7. 3%( 6 /82) and squamous cell carcinoma 9. 8%( 8 /82). Distribution differences,experimental pathology classification result was statistically significant( tongue mucosa χ2= 20. 738,P < 0. 005,palatal mucosal χ2= 15. 003,P < 0. 005). Conclusion This model is an ideal animal model for study on the mechanism of oral cancer occurrence and developmental process.更多还原