【摘要】 采用冷冻干燥技术制备了三维氧化石墨烯/碳纳米管(GO/CNTs)气凝胶,在疏水作用下,该气凝胶对蛋白质表现出良好的吸附性能.在p H=8.0时,GO/CNTs气凝胶对血红蛋白的吸附效率很高,而对白蛋白基本不吸附,从而可实现血红蛋白的选择性吸附分离.在最佳吸附条件下,GO/CNTs气凝胶对血红蛋白的吸附容量高达3793.3 mg/g,是去除高丰度血红蛋白的高效材料.吸附后的血红蛋白可以使用p H=11.5的BrittonRobisin缓冲液有效洗脱,回收率为67%.圆二色光谱结果表明,GO/CNTs气凝胶具有良好的生物相容性,其表面发生的吸附/洗脱过程对血红蛋白的构象基本无影响.将GO/CNTs气凝胶用于人全血样品中血红蛋白的选择性分离,SDS-聚丙烯酰胺凝胶电泳结果表明得到了较高纯度的血红蛋白.更多还原

【Abstract】 3-Dimensional graphene oxide / carbon nanotubes( GO / CNTs) aerogel was prepared via freeze drying technique,and the as-prepared GO / CNTs aerogel exhibits excellent adsorption performance towards protein species under hydrophobic interaction. At p H = 8. 0,high adsorption efficiency of hemoglobin( Hb) was achieved,while virtually no adsorption of serum album was observed. This offers the possibility to realize the selective isolation of Hb from complex matrices. An adsorption capacity up to 3793. 3 mg / g was achieved for the GO / CNTs aerogel,suggesting the great potential of this aerogel in the removal of abundant Hb. The adsorbed Hb could be readily stripped using Britton-Robinson buffer of p H = 11. 5,giving rise to a recovery of67%. Circular dichroism spectra indicated that the adsorption / desorption on the surface of GO / CNTs aerogel poses virtually no effect on the configuration of Hb. The GO / CNTs aerogel is practically applied to the selective isolation of Hb from human blood and SDS-PAGE assays demonstrate high purity of hemoglobin in the eluate.更多还原