【摘要】 采用微波辅助法快速制备以氯霉素为模板分子的分子印迹聚合物.将合成的聚合物作为吸附剂,选择性分离和富集蜂蜜样品中的氯霉素,并结合高效液相色谱-串联质谱法对萃取物进行分析.对合成的分子印迹聚合物进行了表征,考察了聚合物的吸附性能及其选择性,并进行了Scatchard分析.结果表明,合成的聚合物为球形,对氯霉素具有良好的识别能力,最大表观结合量可达0.428 mmol/g.该方法的线性范围为0.5~100 ng/g,相关系数>0.999,蜂蜜中氯霉素的检出限为0.13 ng/g,6种蜂蜜样品中氯霉素的加标回收率范围为88%~93%.更多还原

【Abstract】 The chloramphenicol imprinted polymers were prepared by means of microwave-assisted polymerization and applied for the separation and enrichment of chloramphenicol from honey. The polymers were applied as solid-phase extraction materials to extract and clean up the chloramphenicol in honey,followed by the liquid chromatography-tandem mass spectrometry(LC-MS/MS) analysis. The morphology of polymers was characterized by scanning electron microscope(SEM). The binding property and selectivity of polymers were evaluated and the Scatchard analysis was carried out. The polymers appeared to be spherical in shape and had a good selectivity. The apparent maximum binding amount of the polymers was 427. 7 μmol/g. The detection limit of chloramphenicol was 0. 13 ng/g. The linearity range and correlation coefficient of the method were0. 5—100 ng/g and 0. 999,respectively. The recoveries of six spiked honey samples were in the range of88% —93%. The proposed method has been successfully applied for the detection of chloramphenicol from honey.更多还原