【摘要】 从甜瓜大棚土壤中筛选出菌株FP2,它可有效抑制甜瓜白粉病病原菌、甜瓜枯萎病病原菌、甜瓜疫病病原菌和甜瓜霜霉病病原菌的生长,通过形态学、生理生化和分子生物学研究,FP2被鉴定为枯草芽孢杆菌。为增强该菌株的拮抗能力,用He-Ne激光诱变FP2,得到拮抗活性强且传代最稳定的突变株FP27和FP29,经发酵后将其按1∶1的比例制备成复合接抗菌剂。将复合拮抗菌剂和实验室前期生产的生化黄腐酸菌肥进行复配,首次研发出大棚甜瓜专用的生化黄腐酸复合微生物菌肥。将该菌肥应用于大棚甜瓜的生物防治,结果表明,该菌肥可有效防治大棚甜瓜各类病害的发生,甜瓜长势良好,含糖量高,具有显著的社会效益和经济效益。更多还原

【Abstract】 In this study,an antagonistic strain FP2,which can effectively suppress the melon pathogenic bacteria including Sphaerotheca fuliginea,Fusarium oxysporum,Pytophthora melonis Katsura and Pseudoperonospora cubensis Rostov,was selected from the melon greenhouse soil.Based on phenotypic,physiological,biochemical and phylogenetic(16S rDNA) studies,strain FP2 was identified as Bacillus substilis.In order to enhance its antagonistic capability,the mutation of the strain FP2 was carried out by using repeated He-Ne laser irradiation.After the mutation,6 mutants were screened,among which both mutant FP27 and FP29 showed the strongest antagonist capability against melon pathogen.After the fermentation,the mutant FP27 and FP29 were mixed as 1∶1 ratio to make compound antimicrobial agent.Then,the compound antimicrobial agent were combined with the biofertilizer containing Biochemical fulvic acid(BFA) which were producted by our laboratory previously.For the first time,the compound biofertilizer containing BFA and compound antimicrobial agent,which was specialized in the greenhouse maskmelon biocontrol,wasdeveloped and applied into the biological control of greenhouse melon.The field test results showed that the compound biofertilizer containing BFA and compound antimicrobial community could effectively prevent the melon fromdisease,meanwhile,the melon grew well with high sugar content.更多还原