【摘要】 目的 探讨环氧合酶-2抑制剂对非小细胞肺癌A549细胞血管内皮生长因子(VEGF)表达的影响。方法 加不同浓度塞来昔布与A549肺癌细胞共培养,MTT法检测细胞增殖,50μmol/L塞来昔布作用于A549细胞48 h,RT-PCR方法检测各组细胞的VEGF基因表达水平,Western blot方法检测各组细胞的VEGF蛋白表达。结果 塞来昔布在体外对细胞株A549的生长抑制作用呈剂量、时间依赖性;各组细胞培养48 h后,相比于对照组,RT-PCR方法检测发现塞来昔布组细胞VEGF m RNA表达显著降低(P<0.01),Western blot方法检测发现塞来昔布组细胞VEGF蛋白表达显著降低(P<0.01)。结论 塞来昔布可能通过调节VEGF的表达抑制A549细胞增殖。更多还原

【Abstract】 Objective To study the effect of inhibitor of cyclooxygenase-2 on the expression of vascular endothelial growth factor(VEGF)in A549 cell lines of non-sman cell lung cancers. Methods The proliferation of A549 lung carcinoma cells was measured by MTT assay After A549 cells were treated with different dose celecoxib; After A549 cells were treated with celecoxib 50μmol/L for 48 h, the level of VEGF m RNA was detected RT-PCR, and the expressions of VEGF protein was detected by western blot method. Results Celecoxib could inhibit the proliferation of A549 cell by a time-and dose-dependent manner. Compared with control group, the level of VEGF m RNA was decreased significantly detected by RT-PCR after A549 cells were trealed with celecoxib 50μmol·L-1for 48 h, P<0.01 and the expression of VEGF protein was decreased significantly detected by western blot method, P<0.01.Conclusion celecoxib might inhibite the proliferation of A549 lung carcinoma cells by regulating the expressions of VEGF.更多还原