【摘要】 目的探讨血浆纤溶酶原(Plasminogen)在慢性轻度不可预见性应激(chronic unpredictable mild stress,CUMS)建立抑郁障碍模型中的表达。方法采用SD大鼠建立CUMS抑郁模型(实验组),以不造模的SD大鼠为对照组,免疫组织化学及蛋白质印迹方法分析大鼠海马组织Plasminogen蛋白表达。结果应激后,与对照组比较,实验组体重增长率下降,垂直运动得分和水平运动得分增加,停留时间减少,糖水消耗量减少,差异有统计学意义(P<0.05,P<0.01)。免疫组织化学及蛋白质印迹检测均提示,对照组与实验组大鼠海马区Plasminogen的表达差异有统计学意义(P<0.05,P<0.01)。结论 Plasminogen可能与抑郁障碍的发病机制相关。更多还原

【Abstract】 Objective To investigate plasminogen expression in the rat model of chronic unpredictable mild stress( CUMS). Methods SD rats were used to establish a CUMS depression model. Plasminogen expression in the hippocampus was determined by immunohistochemistry and Western blot. Results Reduced weight gain,increased vertical and horizontal mobility score,decreased staying time and reduced glucose solution consume was revealed in the treatment group compared with the control group after CUMS. Expression of plasminogen in the rat hippocampus after stress was significantly different in both immunohistochemistry and Western blot( P < 0. 05,P < 0. 01). Conclusion Plasminogen may be involved in the pathogenesis of depressive disorders.更多还原