【摘要】 为研究转Ch IFN-α基因百脉根的低温生长特性,对其进行4℃低温离体水培和可溶性蛋白(SP)、丙二醛(MDA)、超氧化物歧化酶(SOD)和脯氨酸(Pro)的测定分析,探讨低温胁迫对转Ch IFN-α基因百脉根的影响及机制。研究发现,25 d低温处理期间,百脉根植株均生长迟缓,野生型(WT)还出现叶片枯黄和茎基部腐烂等冻害现象。转基因百脉根(TP)植株同期的MDA含量比WT低1.03倍(p<0.01),WT的MDA下降幅度仅为TP植株的86%。TP同期的SP、SOD、Pro含量均较WT高(p<0.01),且出现的降幅小。TP同期的SP、SOD和Pro含量分别是WT的1.21倍、1.27倍和1.72倍(p<0.01),WT同期的SP和SOD降幅分别是TP的2.26倍和1.93倍(p<0.01),TP植株的SOD含量峰值比WT提前5 d,TP的Pro在中后期增至WT植株的150%和129%。结果表明,转基因百脉根具有较好的低温耐受性和耐受生理基础,这对其在高寒山区的进一步应用及管理提供了基础依据。更多还原

【Abstract】 For study the growth characteristic of transgenic Ch IFN-α gene lotus plant(Lotus corniculatus L.) in low temperature, the cold-resistant adaptability and regulation mechanism of transgenic plant was studied based on the growth observation and detection of soluble protein(SP), malonadehyde(MDA), superoxide dismutase(SOD) and free proline(Pro), which came from the 4℃ treated lines within 25 days. According to the results transgenic plants with Ch IFN-α gene have better growth compare with the wide type(WT) lines which were gradually scorched on the leaf and decomposed in the end of stem. A higher MDA content of 1.03 times and a drop of 86% were found in WT than TP plants, respectively. In the same growth period, the content of SP、SOD and Pro, in turn, were 1.21,1.27 and 1.72 times(p <0.01) in TP lines than that of the WT. The decline of SP and SOD came from WT lines were 2.26 and 1.93 times of TP(p<0.01). SOD peak content of TP plants moved forward by 5 days compare with WT. Rising Pro contains of TP plants reached 150% and 129% of WT plants in midto late growth stage. Results indicated that Ch IFN-α transgenic lotus plant have good cold-resistence and regulatory power to stress resistance at a biochemical level. This is important for the planting of transgenic Lotus Japonicus in extremely cold areas.更多还原