【摘要】 本研究以四季桂花瓣和叶为材料,采用RT-PCR和RACE技术获得四季桂DXPS基因c DNA全长,并用凝胶定量软件Quanlity-One对四季桂不同季节花瓣和叶片及不同花期花瓣的半定量PCR产物的凝胶图像进行分析。结果表明,四季桂DXPS基因c DNA全长2 185 bp(Gen Bank登录号:KT099324),其中阅读框长1 926 bp,编码641个氨基酸,含有保守功能结构域TPP-DXS、TPP-PYR-DXS-TK-like及Transketolase-C;半定量PCR结果分析表明,四季桂DXPS基因表达量在花朵盛开期最高,花蕾期次之,衰弱期最低;在花瓣中的表达量要明显高于同时期在叶片中的表达量;在花瓣中,12月份DXPS基因的相对表达量最高,3月份次之,在6月份和9月份DXPS相对表达量较低。研究结果初步阐明了DXPS基因在四季桂开花过程中的表达模式,可为深入研究香气合成途径及开展花香植物的分子育种提供科学基础。更多还原

【Abstract】 The full length c DNA sequence of DXPS(Deoxyoxylulose-5-phosphate synthase) gene was cloned from O. fragrans by using RT-PCR and RACE methods. The result showed that the full c DNA of DXPS gene in O.fragrans was 2 185 bp in length(Gen Bank accession No.KT099324), including 1 926 bp of open reading frame. It encoded a protein of 641 amino acid that contains several conserved domains, such as TPP-DXS, TPP-PYR-DXSTK-like and Transketolase-C. The result of semi-quantitative PCR indicated that the relative expression level of DXPS gene from high to low were the blooming stage the buding stage, and the fading stage in order. And it also showed that the relative expression level of DXPS gene in O. fragrans was higher in petals than that of in leaves at the same period. However, the relative level of DXPS gene in petals was the highest on December, followed on March, and lower on June and September. The results of this study illustrated the expression patterns of DXPS gene in O. fragrans flowering process. It may provide scientific basis for intensive study of the aroma synthesis pathway and plant molecular breeding about foral fragrance.更多还原