节点文献

棉花中黄萎病抗性相关microRNA的挖掘及其靶基因分析

Identification and Characterization of mi RNAs and Their Targets Associated with the Verticillium Wilt Resistance in Cotton

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 张玉娟王维陈洁刘聚波夏民旋牛越沈法富

【Author】 Zhang Yujuan;Wang Wei;Chen Jie;Liu Jubo;Xia Minxuan;Niu Yue;Shen Fafu;State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University;

【机构】 作物生物学国家重点实验室,山东农业大学农学院

【摘要】 MicroRNAs(miRNAs)是一类长度约为19~25 nt内源性的、非编码单链小RNA,在植物逆境胁迫应答中具有重要的调节作用。为了获得棉花中黄萎病抗性相关的miRNA,利用生物信息学技术,本研究从Sanger miRNA数据库和已报道的文献中找到64个与棉花黄萎病抗性相关的miRNA,预测和分析了它们的靶基因和启动子,并从中筛选出12个miRNA进行了实时荧光定量PCR分析,为进一步深入研究miRNA的作用机制奠定基础和提高转基因棉花抵抗黄萎病的能力提供新的途径。

【Abstract】 Micro RNAs(miRNAs) are a class of endogenous non-coding small RNAs in length of 19 to 25 nucleotides, which play important roles in regulation of plant stress response. To detect miRNAs related to Verticillium wilt resistance in cotton, 64 potential miRNAs were identified in cotton by mining Sanger miRNA database and the published papaers. Subsequently, the target genes and promoters of these miRNAs were predicted and analyzed by using bioinformatic approaches. Lastly, quantitative real-time PCR assays were performed to analyze the relative expression of 12 miRNAs in cotton roots. This study may pave the way for future in-depth analysis of biological action of miRNAs and provide new information for transgenic breeding for resistance to Verticillium wilt.

【关键词】 miRNA棉花黄萎病靶基因
【Key words】 miRNACottonVerticillium wiltTarget gene
【基金】 国家转基因生物新品种培育科技重大专项课题(2008ZX005-004)资助
  • 【文献出处】 分子植物育种 ,Molecular Plant Breeding , 编辑部邮箱 ,2015年01期
  • 【分类号】S562
  • 【被引频次】8
  • 【下载频次】403
节点文献中: 

本文链接的文献网络图示:

本文的引文网络