【摘要】 植物在长期抵御病原物侵染的进化过程中,通过识别病原相关分子模式(pathogen-associated molecular pattern,PAMPs)激活植物的防卫反应是一个重要的抗病机制。细菌鞭毛蛋白保守的分子特征属于PAMPs,植物可通过识别鞭毛蛋白来感知细菌,从而启动植物自身的免疫系统。这一特性提示鞭毛蛋白在植物抗病育种具有较大的应用前景。本研究利用转基因载体p CAMBIA1300将来源于枯草芽胞杆菌的鞭毛蛋白基因转入水稻,通过转基因植株的分子鉴定和抗性鉴定,筛选到3株苗期高抗稻瘟病的转基因植株。转基因植株不产生类似过敏反应的褪绿斑点。这一结果提示枯草芽胞杆菌鞭毛蛋白在水稻稻瘟病抗性育种中具有一定的应用潜力,获得的转基因抗性植株对不同抗性机制的研究具有一定的意义。更多还原

【Abstract】 An important layer of active defense in plant immunity is the mechanism of pathogen-associated molecular patterns(PAMPs) mediated by cell-surface receptors. Conserved molecular characteristics of bacterial flagellin belong to PAMPs, plants can perceive flagellin to recognize bacteria and activate the immune system.This feature suggests that flagellin has great application prospects in plant breeding for disease resistance. In this study, flagellin gene cloned from Bacillus subtilis was introduced into rice by transgenic vector p CAMBIA1300.Transgenic plants was selected by PCR analysis and disease resistance analysis, finally, data showed transgenic plants possessed highly resistant to rice blast. While transgenic plants have no chlorotic spots on their leaves related to hypersensitivity reactions. Our result suggested that flagellin in Bacillus subtilis can apply in breeding of rice blast and have certain significance for obtaining resistant varieties with different resistance mechanisms.更多还原