【摘要】 目的观察电击死大鼠心脏超微结构及热休克蛋白70(heat shock protein 70,HSP70)、低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)的表达变化,探讨其是否能作为电击死法医学鉴定的依据。方法将72只SD大鼠随机分为电击死组、死后电击组和对照组。通过透射电镜观察电击死大鼠心肌超微结构的改变,并采用免疫组织化学技术观测心肌HSP70和HIF-1α的表达变化。结果电击大鼠心肌细胞肌原纤维断裂,线粒体嵴和膜溶解消失,Z线、M线排列紊乱。HSP70和HIF-1α在电击死组呈强阳性表达,与死后电击组、对照组比较差异有统计学意义(P<0.05)。结论 HSP70和HIF-1α在电击死组表达明显上调,有望作为电击死的诊断参考指标。更多还原

【Abstract】 Objective To observe cardiac ultrastructure and the expression of heat shock protein 70(HSP70)and hypoxia inducible factor-1α(HIF-1α) in electric shock death rats and to explore the application of these indexes as the basis of medical identification in electric shock death. Methods Seventy-two SD rats were randomly divided into electric shock death group, postmortem electric shock group and the control group. The changes of myocardial ultrastructure were observed by transmission electron microscope, and the expressions of myocardial HSP70 and HIF-1α were observed by immunohistochemical technology. Results Myocardial myofibril fracture, mitochondrial cristae and membrane dissolution, and disordered arrangement of Z lines and M lines were observed in electric shock rats. HSP70 and HIF-1αwere strong positive expressions in the electric shock death group, significantly compared with the control and postmortem electric shock groups(P<0.05). Conclusion The expressions of HSP70 and HIF-1αwere obviously increased in electric shock death group, which may be used as the diagnostic indicator of electric shock death.更多还原