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NaCl胁迫对荆芥种子萌发及幼苗抗氧化酶活性的影响

Effect of NaCl stress on germination and seedling’s antioxidant enzymes activity of Nepeta cataria L.

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【作者】 张瑞娥王娟王智颖豆丽君刘小丽

【Author】 ZHANG Rui-e;WANG Juan;WANG Zhi-ying;DOU Li-jun;LIU Xiao-li;School of Biology and Food Engineering,Fuyang Normal University;Engineering Technology Research Center of Anti-aging Chinese Herbal Medicine;

【机构】 阜阳师范学院生物与食品工程学院抗衰老中草药安徽省工程技术研究中心

【摘要】 用纸床培养,通过测定萌发率、胚根长、胚芽长、鲜重等生物量指标及各项生理生化指标的方法探究了不同浓度NaCl(0 mmol/L、25 mmol/L、50 mmol/L、100 mmol/L、150 mmol/L、200 mmol/L)胁迫对特大叶荆芥幼苗种子萌发及幼苗生长的影响。结果表明:NaCl胁迫对荆芥种子萌发率无明显影响,而荆芥根长、芽长、鲜重、子叶展开数和种皮脱落数等均随浓度的增加呈下降趋势,当胁迫浓度≥100 mmol/L时抑制极为显著。SOD和POD活性在25 mmol/L的NaCl胁迫浓度下较对照组升高,而在其后的胁迫浓度下均较对照组低,阴离子氧生成量表现出相应的先下降后上升的趋势。

【Abstract】 By the method of filter paper cultivation,some biomass indexes including germination rate,radical length,germ length,fresh weight and some physiological indexes were studied to explore the influence of NaCl( 0 mmol / L,and 25 mmol / L,and50 mmol / L,and 100 mmol / L,and 150 mmol / L,and 200 mmol / L) stress on seed germination and seedling growth of Nepeta cataria L.. The results showed that effects on seed germination rate was not significant under NaCl( 0 mmol / L,and 25 mmol / L,and 50 mmol / L,and 100 mmol / L,and 150 mmol / L,and 200 mmol / L) stress,while these indexes including root length,bud length,fresh weight,the number of cotyledons opened and seed coat drop decreased with the increasing of NaCl concentration,when the concentrations is higher than 100 mmol / L the inhibition was significant. When concentration of NaCl was 25 mmol / L,SOD and POD activity was higher than the control group,while under subsequent stress,concentration activity was lower than the control group,and the anionic oxygen generation showed a corresponding upward trend after the first drop.

【基金】 安徽省教育厅自然科学一般项目(KJ2013B200);阜阳师范学院自然科学重点项目(2015FSKJ03ZD);阜阳师范学院自然科学一般项目(2012FSKJ03,2013FSKJ18)资助
  • 【文献出处】 阜阳师范学院学报(自然科学版) ,Journal of Fuyang Teachers College(Natural Science) , 编辑部邮箱 ,2015年03期
  • 【分类号】Q945.78
  • 【被引频次】2
  • 【下载频次】138
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