采用光谱法和电化学方法研究了毛蕊异黄酮(CYS)与DNA的作用。CYS与DNA作用后的紫外-可见吸收光谱,表现出一定的减色效应,其荧光光谱则表现出增色效应,表明两者能够发生相互作用。电化学研究表明在p H 4.4的HAc-Na Ac缓冲溶液中,CYS在金电极上有一对氧化还原峰,在0.35~7.04μmol/L和7.04~70.36μmol/L的浓度范围内,CYS的氧化峰电流与其浓度呈良好的线性关系。CYS与DNA作用后,CYS的氧化峰电流显著减小,峰电位发生正移。根据DNA加入前后,峰电流及峰电位的变化,计算了CYS与DNA相互作用前后的动力学参数。
【英文摘要】
The interactions of calycosin( CYS) with DNA were investigated by electrochemical methods coupled with different spectroscopic techniques. According to the spectral measurements,with the increasing amount of DNA added to calycosin,hypochromic and bathochromic shift in the maximum absorption bands of CYS in UVVis as well as the hyperchromic in emission intensity in fluorescence studies were observed,which demonstrate the interaction between CYS and DNA. The gold electrode was used for electrochemical studies...
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