【摘要】 建立了超高效液相色谱-串联质谱法快速检测柑橘中腾毒素、链格孢酚、交链格孢酚单甲醚、交链孢烯和细交链格孢菌酮酸5种链格孢霉毒素的新方法。样品经改进的QuEChERS（快速、简单、廉价、高效、灵活和安全）方法一步完成萃取净化,采用乙腈-甲酸（1.5%）提取,无水MgSO₄和NaCl盐析,以ACQUITY UPLC BEH C₁₈柱为分离柱,用乙腈和0.1%甲酸溶液进行梯度洗脱,电喷雾正离子（ESI⁺）多反应模式监测,超高效液相色谱-串联质谱（UPLC-MS/MS）测定。在本方法条件下,5种链格孢霉毒素在2.0-100μg/L浓度范围内均呈现良好的线性关系（R²>0.9922）,检出限在0.11-0.91μg/kg之间。在5.20和100μg/kg加标水平下,5种毒素的回收率在71%-112%之间,相对标准偏差为1.1%-9.9%,能满足柑橘中链格孢霉毒素检测的要求。更多还原

【Abstract】 A novel method was developed for the rapid determination of five alternaria mycotoxins,alternariol,alternariol monomethyl ether,altenuene,tentoxin and tenuazonic acid,in citrus using ultra-high performance liquid chromatography-tandem mass spectrometry.The sample was prepared using the modified QuEChERS（quick,easy,cheap,effective,rigged,and safe） method to complete the extraction and cleanup steps in one procedure.In this QuEChERS method,sample was extracted with acetonitrile（1.5% formic acid）,then salted out with anhydrous MgSO4 and NaCl,separated on an ACQUITY UPLC BEH C₁₈ with gradient elution by using acetonitrile and 0.1% formic acid aqueous as eluant,and detected by UPLC-MS /MS under negative（ESI⁺） electrospray ionization and MRM models.Under these conditions,five alternaria mycotoxins made a good linearity in the concentration range of 2.0- 100 μg/L with R²>0.9922,and the limits of detection of the instrument were in the range of 0.11-0.91 μg/kg.Three spiked levels of five altenaria mycotoxins at 5,20 and 100 μg/kg were investigated,and the recoveries ranged from 71% to 112% with the relative standard deviations（RSDs） varying from 1.1% to 9.9%,which met the requirement of mycotoxins determination.更多还原