【摘要】 考察了对称型卤代咪唑基离子液体咪唑环上的烷基链长度和不同卤素阴离子(Cl!,Br!,I!)对其光谱性能的影响。实验结果表明,随离子液体咪唑环中烷基链长度增加,离子液体的荧光强度增大;随离子液体中卤素阴离子的电负性降低,其荧光强度减弱。加入血红蛋白可导致咪唑型离子液体的荧光强度增强,且在一定范围内与蛋白质浓度成正比,据此可对血红蛋白进行定量检测,线性范围为0.03~1.0μmol/L,检出限为8 nmol/L。另外,根据不同蛋白质对咪唑基离子液体荧光性能的影响,建立了阵列传感系统用于8种蛋白质的区分与识别,在蛋白质浓度高于500 nmol/L时,识别正确率达到90%以上。更多还原

【Abstract】 Imidazolium ionic liquids( ILs) have extensive applications in various fields. In the present study,the influence of the carbon-chain length of alkyl group on the imidazolium ring and the various halogen anions including Cl!,Br!and I!on the spectral properties of ILs were systematically investigated. We found that the fluorescence intensity of imidazolium ILs increased with the increasing carbon-chain length of alkyl groups,while decreased with the decreasing electronegativity of the halogen anions. The addition of hemoglobin( Hb) into the ILs solutions resulted in the fluorescence enhancement of ILs,thus a quantitative method was developed for Hb detection within a linear concentration range of 0. 03- 1. 0 μmol / L,with a detection limit of 8 nmol / L. Based on the effect of different protein species on the spectral properties of the imidazolium ILs,a sensor array was fabricated for the discrimination of 8 proteins. The discrimination accuracy was found to be > 90% when the protein concentrations were higher than 500 nmol / L.更多还原