【摘要】 建立了超高效液相-二极管阵列(UPLC-DAD)同时测定马陆黄仙合剂中5种成分的方法.采用Waters BEH C18色谱柱(Φ1.7μm,100 mm×2.10 mm),甲醇和体积分数为1%的醋酸水溶液按不同比例梯度洗脱,流速0.61 m L/min,检测波长采用定制波长(0~3 min,232 nm;3~7 min,274 nm;7~10 min,249 nm).一次进样,同时测定了马陆黄仙合剂中芍药苷、黄芩苷、黄芩素、汉黄芩素和甘草酸铵的含量.在测定条件下线性关系良好(r为0.9991~0.999 9),平均回收率为99.70%~100.70%.方法简便、准确、可靠、重复性好,可用于马陆黄仙合剂中5个有效成分的同时测定,也可用于该制剂的质量控制.更多还原

【Abstract】 To a method for the simultaneous quantification of five ingredients in Malu Huangxian Miture is established. Ultra- high performance liquid chromatography- photodiode array detector( UPLC- DAD) was performed on a column of Waters BEH( Φ1. 7 μm,100 mm × 2. 10 mm) at the custom wavelengths including 232 nm for 0 ~ 3 min,274 nm for 3 ~ 7 min,and at 249 nm for 7 ~ 10 min. Methanol- 1 % acetic acid was used as the mobile phase using gradient elution at a flow rate of 0. 61 m L / min. The contents of paeoniflorin,baicalin,baicalein,wogonin and ammonium glycyrrhetate were simultaneously detected by a single injection. Good linear correlation were observed( r = 0. 999 1 ~ 0. 999 9) and the average recovery rates were 99. 70% ~ 100. 70%. The proved method is simple,accurate,reliable and repeatable that is suitable for the quality control of Malu Huangxian Mixture.更多还原