【摘要】 目的:探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)中干扰素诱导基因AIM2的表达及其与疾病活动度的相关性。方法:运用半定量逆转录聚合酶链反应(RT-PCR)技术检测30例SLE患者(SLE组)与25例健康体检者(正常对照组)的AIM2 m RNA表达水平,并且分析它们与尿蛋白、补体C3、补体C4、抗ds-DNA抗体及SLE疾病活动指数(SLEDAI积分)的相关性。结果:1与正常对照组比较,SLE组AIM2 m RNA表达显著增高(P<0.01);2 SLE组尿蛋白阳性患者的AIM2 m RNA表达水平较尿蛋白阴性患者及正常对照组显著增高(P<0.01),尿蛋白阴性组AIM2 m RNA表达水平与正常对照组比较,差异无统计学意义(P>0.05);3 SLE组AIM2 m RNA表达水平与补体C3呈显著负相关(r=-0.623,P<0.05),与抗ds-DNA抗体、SLEDAI积分呈显著正相关(r=0.621、r=0.503,P<0.05),与补体C4不相关(r=-0.426,P>0.05)。结论:SLE患者AIM2 m RNA的表达水平显著增高,并与肾脏损伤、疾病活动度相关。更多还原

【Abstract】 Objective:To investigate the relationship between the expression of interferon-induced gene AIM2 in peripheral blood mononuclear cells(PBMCs) of patients with systemic lupus erythematosus(SLE)and the disease activity.Methods:Semi-quantitative Reverse Transcription Polymerase Chain Reaction(RTPCR) was used to detect the expression levels of AIM2 m RNA of 30 patients with SLE(the SLE group) and 25 healthy person(the normal control group),analyzing their relationship with urinary protein,complements C3 and C4,anti ds-DNA antibody and SLE disease activity index(SLEDAI integral).Results: 1 Compared with the normal control group,the expression of AIM2 m RNA in the SLE group increased significantly(P < 0.01);2 The expressions of AIM2 m RNA in patients with positive urinary protein in the SLE group were significantly higher(P < 0.01) than those with negative urinary protein in the group and all in the normal control group.The expressions of AIM2 m RNA in patients with negative urinary protein had no statistical significance(P > 0.05)from those of the normal control group. 3 The expression levels of AIM2 m RNA in the SLE group had a significant negative correlation with complement C3(r =- 0.623,P < 0.05), an obvious positive correlation with anti ds-DNA antibody and SLEDAI integral(r = 0.621,r = 0.503,P < 0.05) and no relationship with complement C4(r =- 0.426,P > 0.05).Conclusion:The expression levels of AIM2 m RNA in SLE patients significantly increase,which are associated with kidney injury and disease activity.更多还原