【摘要】 水华鱼腥藻是常见的有害水华藻种,但其在磷、光强、温度等生境要素协同作用下的生长动力学鲜见报道.本研究在Lehman模型、Steele模型基础上,设置8个PO3-4-P梯度:0、0.1、0.2、0.5、0.75、1.0、2.5、5.0μmol/L,4个温度水平:15、20、25和30℃和4个光强水平:1000、2000、3000和5000 lx,采取单因素实验方法进行水华鱼腥藻室内纯培养.Monod模型表明水华鱼腥藻最适生长温度和光强条件分别是20℃和3000 lx,最大比生长速率(μmax)和半饱和常数(KS)分别为0.447 d-1和0.081μmol P/L;分别用Lehman模型和Steele模型模拟水华鱼腥藻μmax在不同磷浓度下对连续变化的温度和光强的响应,Lehman动力学模拟结果表明水华鱼腥藻的最适生长温度为21.22±0.98℃,μmax和KS分别为0.421±0.011 d-1和0.055±0.009μmol P/L;Steele模型结果表明μmax和KS分别为0.461±0.010 d-1和0.051±0.009μmol P/L,水华鱼腥藻最适生长光强Ik为2650.93±88.19 lx.更多还原

【Abstract】 Anabaena flos-aquae is one of the common harmful cyanobacteria species. However,its growth kinetics co-impacted by phosphate concentration,light intensity and temperature have rarely been reported. In this paper,eight PO3-4-P concentration gradients( 0,0. 1,0. 2,0. 5,0. 75,1. 0,2. 5 and 5. 0 μmol / L),four water temperatures( 15,20,25 and 30℃) and four light intensities( 1000,2000,3000 and 5000 lx) were selected to carry out single factor batch experiments. A maximum specific growth rate( μmax) was used to indicate algae specific growth rate under optimal growth condition. Half-saturation constant( KS)could be applied to describe affinity of algae to nutrients. Regression results from Monod model showed that Anabaena flos-aquae reached μmaxat 20℃ and 3000 lx. The μmaxand KSwere 0. 447 d-1and 0. 081 μmol P / L,respectively. Regression results based on Lehman and Steele Model that examines the relationship between specific growth rate and continuous changing of temperature as well as light intensity,demonstrated that the optimum light intensity( Ik) and temperature( Topt) were 2650. 93 ± 88. 19 lx and21. 22 ± 0. 98℃,respectively. Besides,the kinetic parameters μmax,KS,and Toptfrom Lehman Model were 0. 421 ± 0. 011 d-1,0. 055 ± 0. 009 μmol P / L and 21. 22 ± 0. 98℃,respectively. In the Steele model,μmax,KSand Ikwere 0. 461 ± 0. 010 d-1,0. 051 ± 0. 009 μmol P / L and 2650. 93 ± 88. 19 lx,respectively.更多还原