【摘要】 目的观察迷迭香酸(RA)对单侧输尿管梗阻(UUO)大鼠肾间质纤维化中TGF-β1、结缔组织生长因子(CTGF)表达的影响。方法结扎大鼠左侧输尿管制作UUO模型,以RA为干预因素,应用免疫组化半定量分析、ELISA法检测肾组织TGF-β1、CTGF蛋白的表达。结果 UUO组、UUO+RA组肾组织TGF-β1、CTGF蛋白的表达水平均明显高于假手术(SOR)组(均P<0.05);与UUO组比较,UUO+RA组TGF-β1、CTGF的表达水平均明显降低(均P<0.05)。结论迷迭香酸具有一定的抗肾间质纤维化作用,其机制可能与抑制TGF-β1、CTGF的表达有关。更多还原

【Abstract】 Objective To observe the effect of rosmarinic acid(RA)on expression of transforming growth factor-β1(TGF-β1)and connective tissue growth factor(CTGF)in the model of rat kidney with unilateral ureteral obstruction(UUO).Methods UUO model was established by ligating the left ureter of the rats,rosmarinic acid was regarded as the interference factors.The protein expression of TGF-β1and CTGF were detected by immunohistochemistry and ELISA.The immunohistochemistry was analyzed semi-quantitative by IPP 6.0.Results Compared with that of group SOR,the expression level of TGF-β1and CTGF in renal tissue of group UUO and group UUO+RA was significantly increased(P<0.05).Compared with group UUO,the expression level of TGF-β1and CTGF in renal tissue of group UUO+RA was significantly reduced(P<0.05).Conclusion Rosmarinic acid has certain resistance to renal interstitial fibrosis,and its mechanism may be related to inhibit the expression of TGF-β1and CTGF.更多还原