The aim of this study was to interfere and repress expression the glycerol 3-phosphate dehydrogenase( GPD1) gene in a specific region by constructing a silencing expression vector PURH-SGPD1. The silencing expression vector containing GPD1 gene,PGK strong promoter and CYC1 terminator. Saccharomyces cerevisiae( S. cerevisiae) SY01 mutant gave 10. 52% less glycerol-3-phosphate dehydrogenase activity,13. 2% lower glycerol production,and 12. 06% higher ethanol production compared with the original strain. Resul...