【摘要】 在凡纳滨对虾育苗生产中,以蔗糖作为添加碳源促进育苗水体形成生物絮团。基于宏基因组测序技术对生物絮团细菌群落结构进行分析。在门、纲和属的水平上,通过检测絮团的细菌物种丰度及优势菌种的结果,表明实验组和对照组基本相同,但所占比例有所差异。其中具有清污作用的变形菌纲在实验组所占比例为16.2%,对照组为14.4%,实验组高于对照组;而作为常见病原菌的假交替单胞菌属和弧菌属,在实验组所占比例分别是1.3%和0%,而在对照组分别为1.4%和0.1%,实验组的病原菌比例低于对照组。本文为生物絮团技术在对虾育苗生产中的应用提供基础资料。更多还原

【Abstract】 During larval rearing of Litopenaeus vannamei,the formation of biofloc in culture pond water could be facilitated after adding sucrose as the main carbohydrate sources. The analysis of biofloc taxonomic composition of metagenomic community showed that the species abundance and dominant bacteria in biofloc were fundamentally the same within experimental and control group in the level of phylum,class and genera,but the proportion of bacteria composition was different. The proportion of Alphaproteobacteria detected in experimental and control groups,which have the ability of removing contaminants,was 16. 2% and 14. 4%,respectively. The proportion of Pseudoalteromonas and Vibrio,which were the common pathogenic bacteria,was 1. 3% and 1. 4% together with 0% and 0. 1% in experimental and control groups. The paper provided the basic data for the application of biofloc technology in the process of shrimp nursery and breeding.更多还原