【摘要】 对获得的苋菜环多巴5-糖基转移酶基因(AmaDOPA5-GT)进行生物信息学和蛋白亚细胞定位分析,并利用染色体步移技术克隆该基因的启动子。亚细胞定位结果表明,AmaDOPA5-GT定位于细胞膜。启动子分析结果表明AmaDOPA5-GT启动子中植物光响应元件多达8个,说明光对该基因的表达影响较大。色素含量测定及实时定量PCR结果显示:暗处理抑制苋菜苷合成和AmaDOPA5-GT表达;黄光和绿光抑制苋菜苷合成但对AmaDOPA5-GT表达影响不大;红光显著抑制苋菜苷合成但却显著诱导AmaDOPA5-GT表达;蓝光显著促进苋菜苷合成和AmaDOPA5-GT表达。本研究可为揭示AmaDOPA5-GT基因在苋菜苷的作用奠定基础并为富含甜菜红色素苋菜苷的苋菜育种提供线索。更多还原

【Abstract】 Bioinformatic characteristics and protein subcellular localization of AmaDOPA5-GT gene in Amaranth tricolor were studied.The chromosome walking technique was applied to clone the gene promoter.The protein was found to be located in the cytomembrane.Eight light-responsive elements were identified in the promoter,suggesting that light might be associated with the regulation of the gene expression.The amaranthin content and quantitative real time PCR showed that darkness suppressed both betalain biosynthesis and AmaDOPA5-GT gene expression;red light also suppressed betalain biosynthesis,but significantly enhanced the gene expression;yellow and green light showed no effect on the gene expression,but suppressed the betalain biosynthesis;and,blue light induced both betalain biosynthesis and the gene expression.The results obtained provided a basis for further exploring the function of AmaDOPA5-GT in betalain biosynthesis,as well as a guideline for breeding betalain-rich amarnath.更多还原