【摘要】 以鄂尔多斯二氧化碳地质储存地的土壤和典型植被小麦为研究对象,通过模拟试验,采用PCR-DGGE（denaturing gradient gel electrophoresis）技术对不同浓度CO₂施加条件下土壤真菌多样性进行了研究。结构表明:土壤CO₂浓度由对照区的990 mg/m³增加到35 990、71 980、107 970 mg/m³时,真菌多样性指数（Shannon’s method）依次为1.609、1.386、1.609、1.609。随着土壤CO₂浓度升高,Chaetomium sp.（毛壳菌属）等真菌丰度显著降低,990 mg/m³条件下Pseudallescheria boydii（波氏假阿利什菌）菌种消失;优势菌群Ascobolus crenulatus（粪盘菌科）具有很高的同源性。土壤CO₂浓度升高至107 970 mg/m³对土壤真菌群落结构有一定影响,但其主要建群种未变;高浓度CO₂对一些真菌生长有刺激作用,对另一些真菌生长有抑制作用甚至会导致土壤真菌遗传基因发生改变;尖端赛多孢子菌Pseudallescheria boydii（波氏假阿利什菌）对CO₂浓度升高反应敏感,可能用于地质封存CO₂泄漏微生物监测与甄别指示菌种。更多还原

【Abstract】 The soil and typical vegetation wheat from the area of geological storage of carbon dioxide in Ordos were selected as the research object. The fungal diversity in different concentrations of CO₂ was investigated by using PCR（Polymerase Chain Reaction）-DGGE（Denaturing Gradient Gel Electrophoresis） and 16Sr-DNA library technology in simulation test. The results showed that the fungal diversity coefficient（P）were 1.609（from control area）,1.386,1.609 and1.609 in turn when the concentration of CO₂ in soil increased from 990 mg/m³ to 35 990 mg/m³,71 980 mg/m³,107 970 mg/m³ respectively. With the increasing concentration of CO₂ in soil,the abundance of fungi such as Chaetomium sp. significantly decreased, and when CO₂ in soil reached 35 990 mg/m³,some species such as Pseudallescheria boydii disappeared.Dominant bacteria such as Ascobolus crenulatus had the higher homology. It can be revealed that high CO₂ concentration of107 970 mg/m³ in soil had a significant impact on the soil fungi community diversity while a weak influence on main bacterial species. The supposed reason is that high concentrations of CO₂ stimulated the growth of some fungi while inhibited others,and may even cause genetic changes in soil fungi;Scedosporium apiospermum such as Pseudallescheria boydii was sensitive to the increase of CO₂ concentration and might be used in microbial monitoring and identifying indicator species of CO₂ leakage from geological storage.更多还原