【摘要】 非变性聚丙烯酰胺凝胶电泳银染技术已广泛用于DNA片段检测。介绍一种本实验室改进的高效省本的非变性聚丙烯酰胺凝胶电泳银染方法,该法将固定和染色两步骤合并,整个银染过程只需16 min即可完成。同时,该法无需使用无水乙醇和冰乙酸,减少了硝酸银、氢氧化钠及甲醛的使用量。更多还原

【Abstract】 A method of silver staining in non-denaturant polyacrylamide gel electrophoresis has widely been used to detect DNA fragments. Here we developed a high-efficiency and low cost method of DNA silver staining in non-denaturant polyacrylamide gel electrophoresis. The whole new procedure of silver staining has combined two steps of fixation and staining into one step and can be completed within 16 min. Furthermore, the new procedure eliminates the use of ethanol and acetic acid and reduces the use amount of silver nitrate, sodium hydroxide and formaldehyde in silver steps.更多还原