【摘要】 近年来,"液态活检"概念异军突起,不仅可以辅助诊断某些类型实体肿瘤,而且能够监测复发,评估疗效及肺癌分子表达,更是无创肿瘤筛查方法。其来源主要分为循环肿瘤细胞(circulating tumor cells,CTCs),循环肿瘤DNA(circulating tumor DNA,ct DNA)等。本文讨论了肺癌领域中CTCs、ct DNA及其他肿瘤标志物的生物学特征、检测方法及临床应用,总结了保证特异性前提下的高敏感度、便于临床应用、可重复性好三个重要评判标准的"液态活检"技术,尤其是肺癌I期敏感度可达67%的靶向PCR CTC技术的应用,以期使"液态活检"真正从科研探索走向临床应用。更多还原

【Abstract】 In recent years, "liquid biopsy" received enormous attentionas a new detecting method. As a noninvasive tumor screening method, the applications of liquid biopsyinclude early detection, monitoring relapse, assessment of therapy and molecule expression in lung cancer. The main source of liquid biopsy comes from circulating tumor cells(CTCs), ct DNA, and so on. This review will explore the biological characteristics, detection technologies and clinical applications of CTCs, ct DNA and other tumor markers in lung cancerand summarize liquid biopsy which in accord with three important criteria of high sensitivity(highspecificity), clinical utility and repeatability, especially a new method of ligand-targeted PCR(LT-PCR) that showed a high sensitivity of 67.2% in stage I lung cancer. We expect that "liquid biopsy" could be really explored from scientific research to clinical application.更多还原