【摘要】 从广东珠三角地区某鸽场的发病鸽群采样,接种10日龄SPF鸡胚后分离到一株病毒,命名为JP株。系列微量血凝和微量血凝抑制试验结果表明,新城疫病毒(NDV)阳性。测序结果显示,该NDV分离株F蛋白裂解位点的氨基酸序列为112 R-R-Q-K-R-F117,具有强毒株序列特点;F基因分型表明,JP株属于基因Ⅵ型。Blast结果显示,JP株F、HN基因序列均与基因Ⅵ型pi/CH/LGD/110208株同源性最高,均达99%。同源性比较发现,F和HN基因、蛋白都与基因Ⅵ型新城疫毒株同源性较高,分别为92.5%~99%、95.5%~99.1%和90.6%~98.7%、91.9%~99%,而与国内常用疫苗株B1、La Sota、Mukteswar等同源性较低,分别为83.2%~85.9%、89.2%~91%和79.3%~83.4%、86%~89.5%。分离的JP株属于基因Ⅵ型,并与国内常用疫苗株存在一定的差异。更多还原

【Abstract】 Some sick pigeons were sampled from pigeon farm in Pearl River Delta region,and used to inoculate 10day-old SPF chicken embryos,and a virus strain named JP was isolated.The positive result of Newcastle disease virus was determined by HA and HI tests.The result of sequence analysis showed that the amino-acid sequence of the fusion protein cleavage site was 112R-R-Q-K-R-F117,a typical sequence of virulent strains.Genetic analysis showed that the isolate belonged to genotype Ⅵ.The result of Blast showed that the sequences of F and HN genes of the isolate have the highest identities with the genotypeⅥ NDV(pi/CH/LGD/110208),both with 99%.The homology analyses of F and HN genes and proteins showed that the isolate were high identities with the genotypeⅥ NDV,with 92.5%-99%,95.5%-99.1%and 90.6%-98.7%,91.9%-99%,respectively,but low identities with the common vaccine strains,such as B1,Mukteswar and La Sota,only with 83.2%-85.9%,89.2%-91%and 79.3%-83.4%,86%-89.5%,respectively,the isolate differs from the common vaccine strains.更多还原