【摘要】 为了解猪雷极氏普罗菲登斯菌(P.rettgeri)的生物学特性、致病性及16SrRNA基因系统进化关系,从发生严重腹泻、血便的哺乳仔猪群的内脏器官中分离到1株病原菌,根据形态特征、培养特性、生化特性、16SrRNA基因序列分析鉴定为P.rettgeri。小鼠攻毒试验证实,该分离菌株对试验小鼠有较强致病力,哺乳仔猪回归试验可复制出与临床上相似的典型症状,并从发病死亡小鼠及哺乳仔猪中分离到攻毒菌株。系统进化分析表明,分离菌株与雷极氏普罗菲登斯菌系统进化关系最为密切,其16SrRNA基因序列与雷极氏普罗菲登斯菌代表菌株的同源性在97.3%~99.6%之间。药敏试验结果表明,分离菌株对头孢哌酮钠、头孢噻肟钠、头孢曲松钠、头孢唑啉、头孢呋肟、头孢吡肟、阿莫西林、链霉素、氨曲南、诺氟沙星、左氟沙星、恩诺沙星、卡那霉素、氨苄青霉素、米诺环素、链霉素、阿米卡星等多数药物敏感。首次报道了雷极氏普罗菲登斯菌可以引起哺乳仔猪严重腹泻,提示在仔猪腹泻中应注意该菌感染的诊断、监测和防控。更多还原

【Abstract】 Investigation of a serious piglet disease with high mortality and typical diarrhea yielded a bacterial isolate identified as Providencia rettgeri based on the morphological characteristics,physiological,biochemical characteristics and 16 Sribosomal DNA sequence analysis.The pathogenicity of this bacterial isolate was confirmed in mice,and the same clinical signs were observed in 5-day-old piglets inoculated with Providencia rettgeri Guangxi 2013 at 5.0×109 CFU.The same bacterial were recovered from the samples collected from died mice and piglets based on bacteria staining or biochemical test results.Phylogenetic analysis showed the isolated strain had the nearest relationship with the P.rettgeri strains,and shared97.3%-99.6% homology of 16 SrRNA with those strains.The drug resistance spectrum of the bacterium was also determined.The results indicated that the isolate is sensitive to 17 antimicrobials out of the 29 antimicrobials tested.The current study is the first to report a serious diarrheal disease in piglets infected with P.reggteri isolate,which should give more attention during surveillance and diagnostics.更多还原