Objective To construct a recombinant lentivirus vector for Wilm's tumor on X chromosome(WTX) gene and establish a colorectal cancer SW620 cell line with stable WTX over-expression. Methods The full length coding region of WTX gene was amplified with PCR, and the amplified fragment was cloned into the lentivirus vector GV387. The recombinant lentivirus vector was transfected in 293 T cells for packaging the virus, which was then transfected into colorectal cancer SW620 cells. The stably transfected cells wer...
WTX(Wilm's tumor on X chromosome,又称FAM123B、AMER1、OSCS)是首个被发现定位于X染色体上的抑癌基因[1]。WTX于2007年首次在《Science》上被报道,并被认为在正常器官发育过程中发挥重要[2];在肿瘤等疾病相关研究方面,WTX先后被证实在Wilms瘤[3],合并有颅骨硬化症的条纹状骨?
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