【摘要】 目的利用二重荧光定量PCR技术,建立一种快速、准确和特异的鉴别鼠疫菌的方法。方法设计和优化两套引物和相应的Taqman探针。靶基因包括染色体上的YPO2088基因和毒力质粒p MT1上的caf1基因。结果实验结果表明,该二重定量PCR是敏感和特异的,敏感性为68.3fg/u L,特异性达到100%。需要很少的样本量,约为1 h得出检测结果。结论该二重荧光定量PCR方法操作简单、快速、敏感和特异。更多还原

【Abstract】 Objective To develop a rapid,sensitive and specific assay for the detection of Yersinia pestis. Methods Two sets of primers and corresponding Taqman probes for Y. pestis were designed and optimized. The target sequences included the YPO2088 gene of the chromosome and caf1 gene on the p MT1 virulence plasmid. Results The results show that this duplex real- time PCR assay using Taq Man chemistry is sensitive and specific,the sensitivity of this assay was determined to be 68. 3fg / u L,this assay was also determined to be 100% specific with strains of Y. pestis and required minimal samples and can yield results in approximately 1hour. Conclusion The duplex real- time PCR method is simple,rapid,highly sensitive,and specific for.Yersinia pestis detection.更多还原