【摘要】 通过SSR标记分析91份油菜品种、骨干亲本和高油自交系,以及作为参考物种的芥菜型油菜、白菜型油菜、埃塞俄比亚芥等8份材料之间的亲缘关系及遗传多样性,为油菜杂种优势利用育种提供理论依据。筛选48对SSR引物对99份参试材料进行扩增,共检测到255个多态性条带。UPGMA聚类将99份材料聚为5组:第Ⅰ组包括89个材料,均为甘蓝型油菜;第Ⅱ组有埃塞俄比亚芥和‘G8白’2个白花材料;第Ⅲ组包括4个芥菜型材料;第Ⅳ组包括3个白菜型材料;第Ⅴ组仅有‘秦芥2008’。第Ⅰ组89个甘蓝型油菜又可分为5个亚组。第i亚组包括43个材料,主要为陕西的恢复系、华中农业大学和浙江材料;第ii亚组包括9个材料,以陕西材料为主;第iii亚组包括12个材料,以贵州和国外材料为主;第iv亚组包括19个材料,主要为中国农科院油料作物所、浙江农科院和安徽的材料;第v亚组包括‘浙油758’、‘147C’等6个材料。而且来自同一育种单位的大部分材料具有较高相似性,这可能是由于育种单位频繁使用少数核心骨干亲本所造成的。更多还原

【Abstract】 In this study,genetic relationship and diversity based on SSR markers were investigated in 91 rapeseed accessions including registered varieties,backbone parents,and high-oil inbred lines,as well as 8references fromBrassica juncea,B.campestris and B.carinata.48 pairs of selected SSR primers from A and C genomes were employed and 255 polymorphic bands were detected.UPGMA clustering showed that99 accessions were divided into 5groups.The largest group,group Ⅰ included 89 B.napus materials.Group Ⅱ only included 2white flower accessions named Ethiopia mustard and G8.GroupⅢ consisted of 4B.juncealandraces.Group Ⅳ were 3 B.campestrislandraces and groupⅤ contained only a mustard named Qinjie 2008,respectively.The 89 B.napusin groupⅠ could be further divided into 5subgroups.Subgroup1 included 43materials,most of which were restorer lines from Shaanxi and materials from Huazhong Agricultural University and Zhejiang.Subgroup 2had 9 materials mainly from Shaanxi.12 materials were included in subgroup 3and most of them were Guizhou and foreign materials.Subgroup 4consisted of 19 materials mainly from Oil Crops Research Institute,CAAS,Zhejiang Academy of Agricultural Sciences,and Anhui.Subgroup 5included 6materials,such as Zheyou 758 and 147C.Most materials from same institutions had a higher similarity.The reason might be that these varieties or lines were bred with limited coreinbred lines.The results could offer helpful information for rapeseed breeding and utilization of heterosis.更多还原