【摘要】 目的探讨维生素E(VE)对苯并[a]芘(benzo[a]pryene,B[a]P)所致雄性SD大鼠神经毒性的拮抗作用。方法将60只SD雄性大鼠随机分为空白、溶剂对照组、B[a]P组及VE低、中、高联合B[a]P处理组,每组10只。连续灌胃30d。通过Morris水迷宫测试大鼠学习记忆能力;观察海马形态学改变以及海马组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷氨酰半胱氨酸合成酶(γ-GCS)活性及谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)、丙二醛(MDA)含量变化。结果行为学结果显示B[a]P组大鼠较对照组平均逃避潜伏期升高,跨台次数和在原平台象限停留时间降低,差异有统计学意义(P<0.01);而VE可改善各指标变化(P<0.01)。此外,海马神经形态学变化提示VE可拮抗B[a]P的损害作用;与对照组比较,B[a]P组大鼠海马组织SOD、GSH-Px、γ-GCS活性以及GSH、GSSG含量下降,MDA含量上升,差异均有统计学意义(P<0.01),且随着VE剂量增加,各指标均逐渐改善。结论 VE对B[a]P导致的神经毒性具有拮抗作用。更多还原

【Abstract】 Objective To explore the effect of vitamin E(VE)against the neurotoxicity of benzo[a]pryene(B[a]P)in male SD rats.Methods A total of 60 male SD rats were randomly assigned to one of the following six groups(n=10/group):the blank control group,vehicle control group,B[a]P group(5 mg/kg),low dose of VE(10 mg/kg)+ B[a]P(5 mg/kg)group,medium dose of VE(50mg/kg)+ B[a]P(5mg/kg)group and high dose of VE(100mg/kg)+ B[a]P(5mg/kg)group.The rats were gavaged with the corresponding dose of B[a]P once a day for 30 days.Morris water maze was used to evaluate the learning and memory performance of rats.Morphological changes in hippocampus were observed.The activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)andγ-glutamylcysteine synthetase(γ-GCS),the contents of glutathione(GSH),oxidized glutathione(GSSG)and malonaldehyde(MDA)in the hippocampus tissue were all examined.Results The results of the behavior tests showed that rats treated with B[a]P exhibited a significantly increased escape latency and a significantly decreased cross-platform times and the residence time on the platform compared with the blank control group and vehicle group(P<0.01).VE treatment could obviously improve the changes of the above index(P<0.01).Besides,the morphological changes in hippocampus suggested that VE could protect against the injury induced by B[a]P.The activities of SOD,GSH-Px andγ-GCS and the contents of GSH,GSSG in rat hippocampus of B[a]P group were significantly decreased,while the content of MDA was significantly increased compared with the blank control group and vehicle control group(P<0.01).Moreover,the above indices were obviously improved with the increase of VE dosage in VE groups.Conclusion VE can play an protective role in B[a]P-induced neurotoxicity.更多还原