【摘要】 目的探讨三维球体胎盘来源间充质干细胞(MSCs)移植对大鼠缺血再灌注损伤脑组织Nogo-A及其受体NgR表达的影响。方法大鼠随机分为假手术组(Sham组)、溶剂对照组(Vehicle组)及治疗组(MSCs组),后两组应用线栓法制备大脑中动脉缺血模型,缺血2h后拔除鱼线再灌注,1d后MSCs组注射三维球体MSCs,Vehicle组注射等量培养液;移植后1、3、7d测定大鼠神经运动功能,取脑组织,应用RT-PCR法、蛋白质印迹法检测Nogo-A、NgR mRNA及蛋白表达。结果与Vehicle组相比,MSCs组大鼠7d神经运动功能有明显改善(P<0.05);移植后1、3、7d MSCs组Nogo-A、NgR mRNA及蛋白水平均较Vehicle组降低(P<0.05)。结论三维球体MSCs移植可改善脑缺血再灌注后神经运动功能,其机制可能与下调脑组织Nogo-A及其受体NgR水平有关。更多还原

【Abstract】 Objective To investigate the effect of transplantation with the three-dimensional spheroid-cultured mesenchymal stem cells(MSCs)on the expression of Nogo-A and NgR in rats with cerebral ischemia-reperfusion injury.Methods The experimental animals were randomly divided into Sham group,Vehicle group and MSCs treated group.The model of focal ischemia-reperfusion in rats was induced by intraluminal middle cerebral artery(MCA)occlusion with a nylon monofilament suture in Vehicle group and MSCs treated group.The fishing line was unpluged for reperfusion 2hafter ischemia and MSCs were transplanted in MSCs treated group one day later.Equivalent medium solution was given to the Vehicle group1 dlater.On the 1st day,3rd day,and 7th day after transplantation,the neuromotor function of the animals was detected.The brain tissue of rats was harvested for RT-PCR detection of Nogo-Aand NgR mRNA expression in the brain tissue of rats,and Western blotting analysis was used to detect the expression of Nogo-A and NgR protein.Results Compared with the Vehicle group,the neuromotor function was significantly improved in MSCs treated group on the 7th day;and the expressions of NogoA and NgR mRNA and protein were significantly down-regulated in MSCs treated group on the 1st day,3rd day,and 7th day after transplantation(P<0.05).Conclusion Transplantation of the three-dimensional spheroid-cultured MSCs can improve the neuromotor function following cerebral ischemia/reperfusion injury,and its mechanism may be associated with down-regulation of Nogo-A and NgR in the brain tissue.更多还原