【摘要】 fol E基因是高叶酸调控基因,来源于细菌,已证明在西红柿等植物中,提高了叶酸含量。本试验采用农杆菌介导的大豆子叶节法,将fol E基因在大豆受体材料Williams82中表达。共侵染了200个外植体,得到34株转化苗。通过对转化苗进行除草剂(135 mg·L-1)涂抹鉴定,获得14株除草剂抗性转化苗。PCR检测结果显示其中12株为阳性。Southern杂交结果表明,fol E全长c DNA已成功插入到大豆基因组中,并得到2个单拷贝株系。更多还原

【Abstract】 The fol E is a key gene of folic acid pathway,which is derived from bacteria. The function of this gene in the improved folate content has been demonstrated in many plants,such as tomato. In this study,we expressed the fol E by employing the soybean cotyledonary nodes method. We co-cultivated about 200 explants,and got 34 transgenic plants. Stock of Basta( 135 mg·L- 1) was painted on half the upper surface of tested soybean leaves,and got 14 transgenic plants. PCR analysis showed that 12 of 14 transgenic plants were positive. Southern blot analysis of PCR-positive plants indicated the integration of the fol E gene into the soybean genome,and we also got two transgenic lines with only one single copy.更多还原