【摘要】 γ-氨基丁酸(GABA)是一种非蛋白质组成成分的氨基酸,具有降血压等多种药理作用。为了更好地开发利用果桑生产中的桑树GABA,分别测定了优良果桑品种红果2号桑树的芽、叶、果,以及枝条、树干、根的皮部和木质部中的GABA含量,结果发现GABA在芽、叶中的含量最高,皮部次之,果和木质部最低。综合考虑GABA在桑树各部位的分布和材料来源,将GABA含量相对较高的桑叶加工成绿茶,并利用响应曲面法对微波辅助提取桑叶绿茶中GABA的工艺条件进行优化,当微波功率为558.89 W和处理时间为11.69 min时,GABA的提取效果最优,验证试验的GABA提取效率检测值与应用回归模型的预测值的符合率为98.52%。研究结果表明,利用果桑生产的副产物桑叶制备桑叶绿茶,采用优化的微波辅助提取条件,可以较为高效地获取GABA等活性物质更多还原

【Abstract】 γ-Aminobutyric acid( GABA) is a kind of non-protein amino acid. It has various pharmacological functions such as lowering blood pressure. In order to better develop and utilize GABA in mulberry,the contents of GABA in different parts of mulberry including bud,leaf,fruit,phloem and xylem of branch,trunk and root from Hongguo 2,the excellent mulberry variety for fruit use,were determined. It was found that the contents of GABA in buds and leaves are the highest,followed by phloem,and the lowest in fruits and xylem. Considering actual distribution of GABA in different parts and material source,mulberry leaves with high GABA content were processed into green tea and microwave-assisted extraction technological conditions for GABA extraction from mulberry-leaf green tea was optimized using response surface methodology. When microwave power was 558.89 W and processing time was 11. 69 min,the extraction efficiency of GABA was the highest. The coincidence rate between detected value of GABA extraction rate in verification test and predicted value calculated by using the regression model was 98. 52%. These results demonstrated that GABA and other active substances can be effectively extracted from mulberry-leaf green tea,which was prepared from the by-product mulberry leaves in mulberry fruits production,through the optimized microwave-assisted extraction technological conditions.更多还原