【摘要】 以枸杞栽培品种宁杞一号和美洲引进野生种L.exsertum为试验材料,采用切根法接种分离自发病枸杞的强致病菌F.oxysporum,研究接种后0~20d枸杞叶片中过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)等抗氧化酶的动态变化。结果显示,接种后L.exsertum的POD,SOD和PAL活性和活性增加量均高于宁杞一号;CAT和PPO活性虽低于宁杞一号,但活性增加量高于宁杞一号,且高酶活持续时间较长,与宁杞一号差异显著(P<0.05)。接种后SOD和PAL活性的高低,POD、CAT、SOD、PPO和PAL活性的增加幅度均可以作为筛选枸杞抗镰孢菌根腐病能力的指标。更多还原

【Abstract】 Used the wolfberries,NingqiⅠcv.and L.exsertumas the testing materials,the studying isolated the strong pathogenic that Fusarium oxysporum with root cutting method from the materials,and studied the change of peroxidase,catalase,superoxide dismutase,polyphenol oxidase as well as phenylalanine ammonialyase of leaves from 0to 20 days after inoculation.The result showed that the enzyme activity,including POD,SOD,PAL and its increment of L.exsertum were higher than that of NingqiⅠcv.The activity of CAT and PPO were lower than that of NingqiⅠcv.,but,the increment of the enzyme activity was higher than that of NingqiⅠcv.and the high enzyme activity had longer time,which had significant difference compared to that of NingqiⅠcv.(P<0.05).The enzyme activity level of SOD and PAL and its increment of POD,CAT,SOD,PPO and PAL after inoculation can be used as a index of screening against root rot with Fusarium of Chinese wolfberry.更多还原